Bos d 11 ELISA 2.0 kit - Single plate pack (EPC-BD11-1)

Plate: Pre-coated with anti-Bos d 11 monoclonal antibody CC11
Vial 1: (white top) Bos d 11 allergen standard
Concentration: 10,000ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody VBIC
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

EPC-BD11-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

Binds to an epitope on bovine Bos domesticus β-casein allergen, Bos d 11.

Binds to an epitope on bovine Bos domesticus β-casein allergen, Bos d 11.

Natural Bos d 11 allergen was prepared in 1% BSA/ 50% glycerol/PBS, pH 7.4.

References:

1.  Luyt D, Ball H, Makwana N, Green MR, Bravin K, et al. BSACI Guideline for the Diagnosis and Management of Cow's Milk Allergy. Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology. 2014.

2.  Fiocchi A, Bouygue GR, Albarini M, Restani P.  Molecular diagnosis of cow’s milk allergy.  Curr Opin Allergy Clin Immunol. 2011 Jun;11(3):216-21.