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Natural Bos d 5 (NA-BD5-1)

  • Natural Bos d 5 (NA-BD5-1)
Allergen: nBos d 5 (β-Lactoglobulin), Cow's milk
Unit: 250µg
Source: Partially purified Bovine milk 
Mol. Wt: 18 kD
Purification:

Column chromatography. Purity on silver stained SDS-PAGE >95%.

Concentration:

See product insert.  Based on OD 280 nm. Extinction coefficient = 0.94

Formulation:

Preservative and carrier-free in endotoxin free phosphate buffered saline, pH 7.4. Filtered through 0.22µm filter.

Storage: Store at -20ºC
Notes: β -Lactoglobulin runs at 18 kD with dimer at 36 kD on non-reducing SDS-PAGE.
PDB:  
Product Resources:

Natural Bos d 5 Certificate of Analysis

Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.
References:  
  1. Alexander LJ, Hayes G, Pearse MJ, Beattie CW, Stewart AF, et al.  Complete sequence of the bovine beta-lactoglobulin cDNA.  Nucleic Acids Res 1989;17(16):6739.
  2. Roth-Walter F, Pacios LF, Gomez-Casado C, Hofstetter G, Roth GA, et al. The major cow milk allergen Bos d 5 manipulates T-helper cells depending on its load with siderophore-bound iron. PLoS One. 2014; 9(8): e104803.
  3. Luyt D, Ball H, Makwana N, Green MR, Bravin K, et al. BSACI Guideline for the Diagnosis and Management of Cow's Milk Allergy. Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology. 2014.
  4. Fiocchi A, Bouygue GR, Albarini M, Restani P.  Molecular diagnosis of cow’s milk allergy.  Curr Opin Allergy Clin Immunol. 2011 Jun;11(3):216-21.
  5. Kuwata K, Hoshino M, Forge V, Era S, Batt CA, Goto Y. Solution structure and dynamics of bovine beta-lactoglobulin A. Protein Sci. 1999 Nov;8(11):2541-5.
File Name Size Added Link
NA-BD5_CoA 221.79KB 23/03/2015 Download
Natural Bos d 5 Certificate of Analysis
  • Product Code: NA-BD5-1
  • Availability: In Stock
  • $625.00

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Tags: milk allergen, food allergen, bos d 5, β-Lactoglobulin, milk,