NBos d 5 ELISA 2.0 kit - Twenty-five plate pack (EPC-NBD5-25)

Description
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ELISA 2.0 kit for measuring native Bos domesticus β-Lactoglobulin allergen, NBos d 5.
Antibodies:		NBD5-1/NBD5-2
Standard Range:		50-0.1ng/mL
Limit of Detection:		0.19ng/mL
Background:		OD<0.08 at 450nm
Coefficient of Determination:		R-squared>0.98
Contents:		Plate: Pre-coated with anti-NBos d 5 monoclonal antibody NBD5-1 Vial 1: (white top) NBos d 5 allergen standard Concentration: 500ng/ml Vial 2: (brown) Biotinylated monoclonal antibody NBD5-2 Vial 3: (blue top) Streptavidin-peroxidase Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid)
Storage:		Store at 2-8°C
Expiry:		See product insert.
Not Provided:		Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required)
Notes:		The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:		EPC-NBD5-25 Certificate of Analysis Validation Performance Data
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Pre-coated Plate:		96-well polystyrene microtiter plate coated with monoclonal antibody NBD5-1 and treated with stabilizing agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody:		NBD5-1
Immunogen:		Bovine β-Lactoglobulin allergen
Isotype:		IgG1
Specificity:		Binds to an epitope on bovine Bos domesticus β-Lactoglobulin allergen, Bos d 5.
Purification:		Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

		Certificate of Analysis
Detection Antibody:		NBD5-2
Immunogen:		Bovine β-Lactoglobulin allergen
Isotype:		IgG1
Specificity:		Binds to an epitope on bovine Bos domesticus β-Lactoglobulin allergen, Bos d 5.
Purification:		Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation:		Biotinylated and titrated for use in ELISA 2.0 at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

		Certificate of Analysis
Allergen Standard:		Natural Bos d 5 allergen was prepared in 1% BSA/ 50% glycerol/PBS, pH 7.4. .
Concentration:		500ng/mL (based on amino acid analysis)
References: 1. Alexander LJ, Hayes G, Pearse MJ, Beattie CW, Stewart AF, et al. Complete sequence of the bovine beta-lactoglobulin cDNA. Nucleic Acids Res 1989;17(16):6739. 2. Roth-Walter F, Pacios LF, Gomez-Casado C, Hofstetter G, Roth GA, et al. The major cow milk allergen Bos d 5 manipulates T-helper cells depending on its load with siderophore-bound iron. PLoS One. 2014; 9(8): e104803. 3. Luyt D, Ball H, Makwana N, Green MR, Bravin K, et al. BSACI Guideline for the Diagnosis and Management of Cow's Milk Allergy. Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology. 2014. 4. Fiocchi A, Bouygue GR, Albarini M, Restani P. Molecular diagnosis of cow’s milk allergy. Curr Opin Allergy Clin Immunol. 2011 Jun;11(3):216-21. 5. Kuwata K, Hoshino M, Forge V, Era S, Batt CA, Goto Y. Solution structure and dynamics of bovine beta-lactoglobulin A. Protein Sci. 1999 Nov;8(11):2541-5.