NBos d 5 ELISA 2.0 kit - Single plate (EPC-NBD5-1)

Plate: Pre-coated with anti-NBos d 5 monoclonal antibody NBD5-1
Vial 1: (white top) NBos d 5 allergen standard
Concentration: 500ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody NBD5-2
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

EPC-NBD5-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

Binds to an epitope on bovine Bos domesticus β-Lactoglobulin allergen, Bos d 5.

Binds to an epitope on bovine Bos domesticus β-Lactoglobulin allergen, Bos d 5.

Natural Bos d 5 allergen was prepared in 1% BSA/ 50% glycerol/PBS, pH 7.4.

References:

1.  Alexander LJ, Hayes G, Pearse MJ, Beattie CW, Stewart AF, et al.  Complete sequence of the bovine beta-lactoglobulin cDNA.  Nucleic Acids Res 1989;17(16):6739.

2.  Roth-Walter F, Pacios LF, Gomez-Casado C, Hofstetter G, Roth GA, et al. The major cow milk allergen Bos d 5 manipulates T-helper cells depending on its load with siderophore-bound iron. PLoS One. 2014; 9(8): e104803.

3.  Luyt D, Ball H, Makwana N, Green MR, Bravin K, et al. BSACI Guideline for the Diagnosis and Management of Cow's Milk Allergy. Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology. 2014.

4.  Fiocchi A, Bouygue GR, Albarini M, Restani P.  Molecular diagnosis of cow’s milk allergy.  Curr Opin Allergy Clin Immunol. 2011 Jun;11(3):216-21.

5.  Kuwata K, Hoshino M, Forge V, Era S, Batt CA, Goto Y. Solution structure and dynamics of bovine beta-lactoglobulin A. Protein Sci. 1999 Nov;8(11):2541-5.