Recombinant Der p 23 (RP-DP23-1)

Description
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Allergen:	rDer p 23 (Dermatophagoides pteronyssinus allergen 23.0101), house dust mite
Unit:	250µg
Source:	Pichia pastoris
Mol. Wt:	8 kD (rDer p 23 migrates at 16-18kD, see note)
Purification:	Multi-step chromatographic purification. Purity > 95 % by silver stained SDS PAGE.
Concentration:	See product insert.
Formulation:	Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Sterile filtered.
Storage:	Store at -20ºC. Avoid freeze-thaw cycles.
Notes:	1. rDer p 23.0101 has a C-terminal 6xHis-tag. 2. rDerp 23.0101 migrates at 16-18kD on SDS-PAGE under reducing conditions. However, the calculated and actual molecular mass is 8kD. (Weghofer, M. et al. J Immunology 190,2013)

Product Resources:	Der p 23.0101 CoA
Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.
References:
Weghofer M., et al. 2013. Identification of Der p 23, a peritrophin-like protein, as a new major Dermatophagoides pteronyssinus allergen associated with the peritrophic matrix of mite fecal pellets. J. Immunol. 2013; 190: 3059–3067. Banerjee S, et al. Conversion of Der p 23, a New Major House Dust Mite Allergen, into a Hypoallergenic Vaccine. J Immunol:2014;192(10)4867-4875. Soh W.T., et al. The House Dust Mite Major Allergen Der p 23 Displays O-Glycan-Independent IgE Reactivities but No Chitin-Binding Activity. Int Arch Allergy Immunol 2015;168:150-160. Mueller G., et al. Structural, Serological, and Genomic Analyses of the Major Mite Allergen Der p 23. Clin Exp Allergy. 2016 Feb;46(2):365-76.