Der p 2 ELISA 2.0 kit - Twenty-five plate (EPC-D2-25)

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ELISA 2.0 kit for measuring Dermatophagoides spp. Group 2 allergen, Der p 2 *Not recommended for allergen quantification in samples that contain multiple house dust mite species
Antibodies:		1D8/7A1
Standard Range:		25-0.05ng/mL
Limit of Detection:		0.2ng/mL
Background:		OD<0.08 at 450nm
Coefficient of Determination:		R-squared>0.98
Contents:		Plate: Pre-coated with anti-Der p 2 monoclonal antibody 1D8 Vial 1: (white top) Der p 2 allergen standard Concentration: 250ng/ml Vial 2: (brown) Biotinylated monoclonal antibody 7A1 Vial 3: (blue top) Streptavidin-peroxidase Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid)
Storage:		Store at 2-8°C.
Expiry:		See product insert.
Not Provided:		Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required)
Notes:		The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:		EPC-D2-25 Certificate of Analysis Validation Performance Data
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Pre-coated Plate:		96-well polystyrene microtiter plate coated with monoclonal antibody 1D8 and treated with stabilizing agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody:		1D8 (clone 1D8 C4 D8)
Immunogen:		Der p 2
Isotype:		Mouse IgG2A
Specificity:		Binds to a common epitope on mite Dermatophagoides spp. Group 2 allergens, Der p 2 and Der f 2.
Purification:		From ascites by ammonium sulphate precipitation and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

Detection Antibody:		7A1 (clone 7A1 H1 G3)
Vial:		Brown Cap; 100µl
Immunogen:		Der f 2
Isotype:		Mouse IgG1
Specificity:		Binds to a common epitope on mite Dermatophagoides spp Group 2 allergens (Der f 2 and Der p 2).
Purification:		Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation:		Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

Allergen Standard:		Purified natural Der p 2 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration:		250ng/mL (based on amino acid analysis)
References:
1. Luczynska CM, Arruda LK, Platts-Mills TA, Miller JD, Lopez M, Chapman MD. A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f I. J Immunol Methods 1989; 118(2):227-235.
2. Custovic A, Taggart SC, Francis HC, Chapman MD, Woodcock A. Exposure to house dust mite allergens and the clinical activity of asthma. J Allergy Clin Immunol 1996; 98(1):64-72.
3. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24.
4. Sawyer G, Kemp T, Shaw R et al. Biologic pollution in infant bedding in New Zealand: high allergen exposure during a vulnerable period. J Allergy Clin Immunol 1998; 102(5):765-770.
5. Arbes SJ, Jr., Cohn RD, Yin M et al. House dust mite allergen in US beds: results from the First National Survey of Lead and Allergens in Housing. J Allergy Clin Immunol 2003; 111(2):408-414.
6. Gruchalla RS, Pongracic J, Plaut M et al. Inner City Asthma Study: relationships among sensitivity, allergen exposure, and asthma morbidity. J Allergy Clin Immunol 2005; 115(3):478-485.
7. Zock JP, Heinrich J, Jarvis D et al. Distribution and determinants of house dust mite allergens in Europe: the European Community Respiratory Health Survey II. J Allergy Clin Immunol 2006; 118(3):682-690.
8. Jarvis D, Zock JP, Heinrich J et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol 2007; 119:697-704.
9. Earle CD, King EM, Tsay A et al. High-throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol 2007; 119(2):428-433.