Blo t 5 ELISA 2.0 kit - Single plate (EPC-BT5-1)

Description
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ELISA 2.0 kit for measuring Blomia tropicalis allergen, Blo t 5.
Antibodies:		pAb/pAb
Standard Range:		30-0.06ng/mL
Limit of Detection:		0.12ng/mL
Background:		OD<0.08 at 450nm
Coefficient of Determination:		R-squared>0.98
Contents:		Plate: Pre-coated with anti-Blo t 5 purified polyclonal antibody Vial 1: (white top) Blo t 5 allergen standard Concentration: 300ng/ml Vial 2: (brown) Biotinylated purified polyclonal antibody Vial 3: (blue top) Streptavidin-peroxidase Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid)
Storage:		Store at 2-8°C.
Expiry:		See product insert.
Not Provided:		Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required)
Notes:		The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:		EPC-BT5-1 Certificate of Analysis Validation Performance Data
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Pre-coated Plate:		96-well polystyrene microtiter plate coated with purified polyclonal antibody and treated with stabilizing agent. Sealed in foil pouch with desiccant.
Polyclonal Antibody:		Purified rabbit polyclonal IgG
Immunogen:		Blo t 5
Isotype:		Multiple
Specificity:		Binds to an epitope present on dust mite Blomia Tropicalis allergen, Blo t 5.
Purification:		Produced in vitro and purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE.

Detection Antibody:		Purified rabbit polyclonal IgG
Immunogen:		Blo t 5
Isotype:		Multiple
Specificity:		Binds to an epitope present on dust mite Blomia Tropicalis allergen, Blo t 5.
Purification:		Produced in vitro and purified by affinity chromatography. Single heavy and light chain bands on SDS-PAGE.
Biotinylation:		Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

Allergen Standard:		Recombinant Blo t 5 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration:		300ng/mL (based on amino acid analysis)
References:
1. Arruda LK, Rizzo MC, Chapman MD, Fernandez-Caldas E, Baggio D, Platts-Mills TAE, Naspitz CK. Exposure and sensitization to dust mite allergens among asthmatic children in Sno Paulo, Brazil. Clinical Exp Allergy 1991; 21:433-439. 2. Vailes LD, Rizzo MC, Naspitz CK, Montealegre F, Fernandez-Caldas E, Platts-Mills TAE, Chapman MD, Arruda LK. Analysis of IgE antibody responses to D. pteronyssinus and Blomia tropicalis using recombinant group 5 allergens. J Allergy Clin Immunol 1996; 97:213 (Abstract). 3. Arruda LK, Vailes LD, Platts-Mills TAE, Fernandez-Caldas E, Montealegre F, Lin KL, Chua KY, Rizzo MC, Naspitz CK, Chapman MD. Sensitization to Blomia tropicalis in patients with asthma and identification of allergen Blo t 5. Amer J Resp Crit Care Med 1997; 155:343-350.