Der p 23 ELISA 2.0 kit - Single plate (EPC-DP23-1)

Description
Downloads (1)

ELISA 2.0 kit for measuring D. pteronyssinus allergen, Der p 23.
Antibodies:		pAb/7A8
Standard Range:		50-0.1ng/mL
Limit of Detection:		0.39ng/mL
Background:		OD<0.08 at 450nm
Coefficient of Determination:		R-squared>0.98
Contents:		Plate: Pre-coated with purified rabbit polyclonal anti Der p 23 antibody Vial 1: (white top) Der p 23 allergen standard Concentration: 500ng/ml Vial 2: (brown) Biotinylated monoclonal antibody 7A8 Vial 3: (blue top) Streptavidin-peroxidase Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid)
Storage:		Store at 2-8°C.
Expiry:		See product insert.
Not Provided:		Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required)
Notes:		The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:		EPC-DP23-1 Certificate of Analysis Validated Performance Data
For research and commercial use in vitro: not for human in vivo or therapeutic use. License Agreement: Indoor Biotechnologies has executed a patent license agreement with Biomay AG (Vienna, Austria), which covers commercial sale of kits for measurement of Der p 23 by immunoassay.
		Certificate of Analysis
Pre-coated Plate:		96-well polystyrene microtiter plate coated with purified rabbit polyclonal anti Der p 23 antibody and treated with stabilizing agent. Sealed in foil pouch with desiccant.
Polyclonal Antibody:		Purified rabbit polyclonal antiserum
Immunogen:		Der p 23
Isotype:		Multiple
Specificity:		Binds to epitope(s) on D. pteronyssinus allergen, Der p 23. Minor cross-reactivity with Der f 23.
Purification:		Affinity chromatography

Detection Antibody:		7A8
Immunogen:		Der p 23
Isotype:		Mouse IgG1/kappa
Specificity:		Binds to epitope(s) on D. pteronyssinus allergen, Der p 23. Minor cross-reactivity with Der f 23.
Purification:		Produced in cell culture and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation:		Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

Allergen Standard:		Recombinant Der p 23 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration:		500ng/mL (based on amino acid analysis)
References:
1. Weghofer M et al. Identification of Der p 23, a peritrophin-like protein, as a new major Dermatophagoides pteronyssinus allergen associated with the peritrophic matrix of mite fecal pellets. J Immunol 2013; 190: 3059-67.
2. Mueller GA, et al. Serological, genomic and structural analyses of the major mite allergen Der p 23. Clin Exp Allergy 2016. 46(2): 365-76.
3. Posa D, Perna S, Resch Y et al. Evolution and predictive value of IgE responses toward a comprehensive panel of house dust mite allergens during the first 2 decades of life. J Allergy CLin Immunol 2017; 139: 541-9.
4. Zidarn M, Robic M, Krivec A et al. Clinical and immunological differences between asymptomatic HDM-sensitized and HDM-allergic rhinitis patients. Clin Exp Allergy 2019; 49: 808-18.