Natural Der p 1 Molecular Reference Standard (MRS-NDP1)

From spent mite culture by multi-step affinity chromatography. 

Prior to lyophilization, natural Der p 1 was adjusted to 50 mM volatile ammonium bicarbonate with 3% trehalose. 

The Natural Der p 1 MRS is an Inbio^TM product.

• Allow vial to reach room temperature before use
• Tap vial gently to collect all material at the bottom
• Using a sterile syringe reconstitute the MRS to desired concentration by injecting a suitable volume of a buffer of choice (e.g. PBS, pH 7.4 or 1% BSA/50% glycerol/PBS, pH 7.4). 
• Mix by gently swirling the vial until content is completely dissolved. 
• Adding 1ml of buffer will result in a Der p 1 concentration of 10,000ng/ml. 

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2. Wan H, Winton HL, Soeller C, Tovey ER, Gruenert DC, Thompson PJ, Stewart GA, Taylor GW, Garrod DR, Cannell MD, Robinson C.  Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions.  J Clin Invest 1999; 104:123-133.
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