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Asp f 1 ELISA 2.0 kit - Single plate (EPC-AF1-1)
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ELISA 2.0 kit for measuring Aspergillus fumigatus allergen, Asp f 1. |
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| Antibodies: | 4A6/pAb | |||||
| Standard Range: | 40-0.08ng/mL | |||||
| Limit of Detection: | 0.32ng/mL | |||||
| Background: | OD<0.08 at 450nm | |||||
| Coefficient of Determination: | R-squared>0.98 | |||||
| Contents: | Plate: Pre-coated with anti-Asp f 1 monoclonal antibody 4A6 Vial 1: (white top) nAsp f 1 allergen standard Concentration: 400ng/ml Vial 2: (brown) Rabbit anti-Asp f 1 Vial 3: (blue top) Peroxidase conjugated Goat anti. Rabbit IgG Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid) |
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| Storage: | Store at 2-8°C. | |||||
| Expiry: | See product insert. | |||||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required) |
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| Notes: | The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. |
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| Product Resources: | ||||||
For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis | ||||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 4A6 and treated with stabilizing agent. Sealed in foil pouch with desiccant. |
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| Monoclonal Antibody: | 4A6 | |||||
| Immunogen: | Asp f 1 | |||||
| Isotype: | Mouse IgG1 | |||||
| Specificity: | Binds to a common epitope on Aspergillus fumigatus allergen, Asp f 1. | |||||
| Purification: | Produced in tissue culture and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. | |||||
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| Detection Antibody: | Polyclonal antibody raised against partially purified Asp f 1 (1,2). | |||||
| Vial: | Brown Cap; 100µl | |||||
| Specificity: | The pAb contains IgG antibodies to Asp f 1 as well as IgG antibodies to other Aspergillus allergens. | |||||
| Activity: | The pAb is in phosphate buffered saline containing 1%BSA 50%glycerol PBS, pH 7.4. The pAb has been 0.22µm filtered and should be diluted 1/1000 for use in Asp f 1 ELISA. | |||||
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| Allergen Standard: | Naturally purified Asp r 1 prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4 | |||||
| Concentration: | 400ng/mL
The Asp f 1 concentration was determined by amino acid analysis. Asp f 1 and Asp r 1 (mitogillin) are 95% homologous (differing by 4 amino acid residues) and are antigenically and functionally indistinguishable (1). |
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| References: | ||||||
| 1. Arruda, LK, Platts-Mills, TAE, Fox, JW and Chapman, MD (1990). Aspergillus fumigatus allergen I, a major IgE binding protein, is a member of the mitogillin family of cytotoxins. J. Exp. Med., 172:1529-1532. 2. Arruda LK, Platts-Mills TAE, Longbottom JL, Chapman MD (1992). Aspergillus fumigatus: Identification of 16kd, 18kd and 45kd antigens recognized by human IgG and IgE antibodies and murine monoclonal antibodies. J. Allergy Clin. Immunol. 89:1166-1176. 3. Arruda LK, Mann BJ, Chapman MD (1992). Selective expression of a major allergen and cytotoxin, Asp f 1, in Aspergillus fumigatus. J. Immunol. 149:3354- 3359. 4. Sporik RB, Arruda LK, Woodfolk J, Chapman MD, Platts-Mills TAE (1993). Environmental exposure to Aspergillus fumigatus allergen 1 (Asp f 1). Clin. Exp. Allergy 23:326-331. 5. El-Dahr JM, Fink R, Selden R, Arruda LK, Platts-Mills TAE, Heymann PW (1994). Development of immune responses to Aspergillus at an early age in children with cystic fibrosis. Am. J. Respir. Crit. Care Med. 150:1513-1518. 6. Vailes LD, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman MD (2001). Quantitation of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J. Allergy Clin. Immunol. 107:641-646. 7. Chapman MD (2006). Challenges associated with indoor moulds: Health effects, immune response and exposure assessment. Medical Mycology. 44:S29-S32. |
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| (For complete reference list see EPC-AF1 Certificate of Analysis) | ||||||
| File Name | Size | Added | Link |
| EPC-AF1-X_CoA | 1.12MB | 28/04/2020 | Download |
| Certificate of Analysis for Asp f 1 ELISA 2.0 kit | |||
- Product Code: EPC-AF1-1
- Availability: In Stock
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$545.00
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Tags: AF1, ELISA 2.0, mold, pre-coated plate, Asp f 1, Aspergillus fumigatus
