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Ara h 1 ELISA 2.0 kit - Twenty-five plate (EPC-AH1-25)
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ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 1. |
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| Antibodies: | 2C12/2F7 | ||||||
| Standard Range: | 2,000-4.0 ng/mL | ||||||
| Limit of Detection: | 31.5 ng/mL | ||||||
| Background: | OD<0.08 at 450nm | ||||||
| Coefficient of Determination: | R-squared >0.98 | ||||||
| Contents: |
Plate: Pre-coated with anti-Ara h 1 monoclonal antibody 2C12 |
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| Storage: | The ELISA 2.0 kit should be stored at 2-8°C. | ||||||
| Expiry: | See product insert. | ||||||
| Not Provided: |
Type I ultrapure water or 18.2MΩ de-ionized water |
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| Notes: | The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. |
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| Product Resources: | |||||||
For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis | |||||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 2C12 and treated with stabilizing agent. Sealed in foil pouch with desiccant. |
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| Monoclonal Antibody: | 2C12 | ||||||
| Immunogen: | Ara h 1 | ||||||
| Isotype: | Mouse IgG1 | ||||||
| Specificity: | Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1. | ||||||
| Purification: | Produced in vitro by BioVectra dcl bioreactors and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. | ||||||
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| Certificate of Analysis | |||||||
| Detection Antibody: | 2F7 | ||||||
| Immunogen: | Ara h 1 | ||||||
| Isotype | Mouse IgG1 | ||||||
| Specificity: | Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1. | ||||||
| Activity: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free. | ||||||
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| Certificate of Analysis | |||||||
| Allergen Standard: | Purified natural Ara h 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. |
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| Concentration/Calibration: | 20,000 ng/mL (based on amino acid analysis) | ||||||
| References: | |||||||
| 1. Pomés A, Helm RM, Bannon GA, Burks AW, Tsay A, Chapman MD. Monitoring peanut allergen in food products by measuring Ara h 1. J Allergy Clin Immunol 2003;111:640-5. | |||||||
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2. Pomés A, Vinton R, Chapman MD. Peanut allergen (Ara h 1) detection in foods containing chocolate. J Food Prot. 2004 Apr:67 (4):793-8. 3. Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6. 4. Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24. |
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| File Name | Size | Added | Link |
| EPC-AH1-X_CoA | 334.26KB | 28/04/2020 | Download |
| Certificate of Analysis for Ara h 1 ELISA 2.0 | |||
- Product Code: EPC-AH1-25
- Availability: In Stock
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$9,095.00
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Tags: Ara h 1, ELISA 2.0, five pre-coated plate,
