Ara h 2 ELISA 2.0 kit - Single plate (EPC-AH2-1)

Description
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ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 2.
Antibodies:		1C4/pAb
Standard Range:		250-0.5 ng/mL
Limit of Detection:		2.0ng/mL
Background:		OD<0.1 at 450nm
Coefficient of Determination:		R-squared>0.98
Contents:		Plate: Pre-coated with anti-Ara h 2 monoclonal antibody 1C4 Vial 1: (white top) Ara h 2 allergen standard Concentration: 2,500 ng/ml Vial 2: (brown) Rabbit anti Ara h 2 antibody Vial 3: (blue top) Peroxidase-conjugated Goat Anti-Rabbit Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid)
Storage:		The ELISA 2.0 kit should be stored at 2-8°C.
Expiry:		See product insert.
Not Provided:		Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required)
Notes:		The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:		EPC-AH2-1 Certificate of Analysis
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Pre-coated Plate:		96-well polystyrene microtiter plate coated with monoclonal antibody 1C4 and treated with stabilizing agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody:		1C4
Immunogen:		Ara h 2
Isotype:		Mouse IgG1
Specificity:		Binds to a species specific epitope present on Arachis hypogaea allergen, Ara h 2.
Purification:		Produced in ascites and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.

		Certificate of Analysis
Detection Antibody:		Rabbit polyclonal antiserum
Immunogen:		Polyclonal rabbit antiserum raised against natural purified Ara h 2
Isotype		Multiple
Specificity:		The antiserum contains IgG antibodies to peanut Arachis hypogaea allergen, Ara h 2.
Activity:		Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.

		Certificate of Analysis
Allergen Standard:		Purified natural Ara h 2 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:		2,500 ng/mL (based on amino acid analysis)
References:
1. Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.
2. Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.