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Ara h 8 ELISA 2.0 kit - Five plate (EPC-AH8-5)

  • Ara h 8 ELISA 2.0 kit - Five plate (EPC-AH8-5)

ELISA 2.0 kit for measuring Arachis hypogaea allergen, Ara h 8.
Antibodies: 4G6/pAb
Standard Range: 100-0.2ng/mL
Limit of Detection: 0.78ng/mL
Background: OD<0.08ng/ml at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plates: Pre-coated with anti-Ara h 8 monoclonal antibody 4G6
Vial 1: (white top) Ara h 8 allergen standard
Concentration: 1,000 ng/ml
Vial 2: (brown) Rabbit polyclonal antiserum
Vial 3: (blue top) Peroxidase-conjugated Goat Anti-Rabbit
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-AH8-5 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
monoclonal antibody 4G6 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 4G6
Immunogen: Ara h 8
Isotype: Mouse IgG1
Specificity: Binds to an epitope present on peanut allergen, Arachis hypogaea, Ara h 8. Minimal cross-reactive with structurally-homologous birch pollen allergen, Bet v 1.
Purification: Produced in cell culture and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: Rabbit polyclonal antiserum
Immunogen: Ara h 8
Isotype Multiple
Specificity: The antiserum contains IgG antibodies to Ara h 8. Minimal cross-reactivity with structurally-homologous to birch pollen allergen, Bet v 1.
Activity: The antiserum has been titrated for use in ELISA at 1/1,000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Recombinant Ara h 8 prepared in 1% BSA/50%
glycerol/PBS, pH 7.4.
Concentration/Calibration: 1,000ng/mL (based on amino acid analysis)
References:  
 
 
 
 
File Name Size Added Link
EPC-AH8-X_CoA 263.18KB 28/04/2020 Download
Certificate of Analysis for Ara h 8 ELISA 2.0 kit
  • Product Code: EPC-AH8-5
  • Availability: In Stock

  • $1,985.00

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Tags: Ara h 8, ELISA 2.0, pre-coated plate,