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Amb a 1 ELISA 2.0 kit - Single plate (EPC-AM1-1)

  • Amb a 1 ELISA 2.0 kit - Single plate (EPC-AM1-1)

New Formulation!

IMPORTANT: 2018 Amb a 1 Immunoassay letter

ELISA 2.0 kit for measuring Ambrosia artemisiifolia allergen, Amb a 1.
Antibodies: 2B6/4H7
Standard Range: 200-0.4 ng/mL
Limit of Detection: 0.78 ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Amb a 1 monoclonal antibody 2B6
Vial 1: (white top) Amb a 1 allergen standard
Concentration: 2000 ng/ml
Vial 2: (brown) anti-Amb a 1 antibody 4H7
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration
purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-AM1-5 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plates coated with
monoclonal antibody 2B6 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 2B6
Immunogen: Amb a 1 purified from Short Ragweed pollen
Isotype: Mouse IgG1/Kappa
Specificity: Binds to an epitope on Short Ragweed (Ambrosia artemisiifolia) allergen, Amb a 1 (Isoform specificity not determined). Minor cross-reactivity with Western, Giant/Tall and False Ragweed species.
Purification: Produced in cell culture and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: 4H7
Immunogen: Amb a 1 purified from Short Ragweed pollen
Isotype Mouse IgG1/Kappa
Specificity: Binds to an epitope on Short Ragweed (Ambrosia artemisiifolia) allergen, Amb a 1 (Isoform specificity not determined). Minor cross-reactivity with Western, Giant/Tall and False Ragweed species.
Activity: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Purified natural Amb a 1 prepared in 1% BSA/30%
glycerol/PBS, pH 7.4.
Concentration/Calibration: 2,000 ng/mL (based on amino acid analysis)
References:  
 
 
 

 

 
File Name Size Added Link
EPC-AM1-X_CoA 238.65KB 28/04/2020 Download
Certificate of Analysis for Amb a 1 ELISA 2.0 kit
  • Product Code: EPC-AM1-1
  • Availability: In Stock

  • $545.00

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Tags: Amb a 1, ELISA 2.0, pre-coated plate,