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Bla g 5 ELISA 2.0 kit - Five plate (EPC-BG5-5)
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ELISA 2.0 kit for measuring Blattella germanica allergen, Bla g 5. |
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| Antibodies: | 17B12/pAb | |||
| Standard Range: | 250-0.5 ng/mL | |||
| Limit of Detection: | 1.95ng/mL | |||
| Background: | OD<0.1 at 450nm | |||
| Coefficient of Determination: | R-squared>0.98 | |||
| Contents: | Plates: Pre-coated with anti-Bla g 5 monoclonal antibody 17B12 Vial 1: (white top) rBla g 5 allergen standard Concentration: 2500 ng/ml Vial 2: (brown) Rabbit anti Bla g 5 antibody Vial 3: (blue top) Peroxidase-conjugated Goat Anti-Rabbit Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid) |
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| Storage: | Store at 2-8°C | |||
| Expiry: | See product insert. | |||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required) |
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| Notes: | The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website |
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| Product Resources: |
EPC-BG5-5 Certificate of Analysis |
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For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis | ||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 17B12 and treated with stabilizing agent. Sealed in foil pouch with desiccant |
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| Monoclonal Antibody: | 17B12 (clone 10A6 F5 E1) | |||
| Immunogen: | Bla g 5 | |||
| Isotype: | Mouse IgG | |||
| Specificity: | Binds to an epitope on cockroach Blattella germanica allergen, Bla g 5. | |||
| Purification: | Produced in vitro and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. | |||
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| Certificate of Analysis | ||||
| Detection Antibody: | Rabbit polyclonal antiserum | |||
| Immunogen: | Recombinant Bla g 5 | |||
| Isotype: | Multiple | |||
| Specificity: | The antiserum contains IgG antibodies to cockroach Blattella germanica allergen, Bla g 5 and does not react with Bla g 2. | |||
| Activity: | Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free. | |||
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| Certificate of Analysis | ||||
| Allergen Standard: | Purified recombinant Bla g 5 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. |
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| Concentration: | 2500 ng/mL (based on amino acid analysis) | |||
| References: | ||||
| 1. Pollart SM, Smith TF, Morris E, Gelber LE, Platts-Mills TAE, Chapman MD. Environmental exposure to cockroach allergens: analysis with monoclonal Antibody based enzyme immunoassays. J Allergy Clin Immunol 87:505- 510, 1991. | ||||
| 2. Gelber LE, Seltzer LH, Bouzoukis JK, Pollart SM, Chapman MD, Platts-Mills TAE. Sensitization ad exposure to indoor allergens as risk factors for asthma among patients presenting to hospital. Amer Rev Respir Dis 147:573-578, 1993. | ||||
| 3. Rosenstreich DL, Eggleston P, Kattan M, Baker D, Slavin RG, Gergen P, Mitchell H, McNiff-Mortimer K, Lynn H, Ownby D, Malveaux F. The role of cockroach allergy and exposure to cockroach allergen in causing morbidity among inner-city children with asthma. New Eng J Med 336:1356-1363, 1997. | ||||
| 4. Arruda LK, Vailes LD, Ferriani VP, Santos AB, Pomés A, Chapman MD. Cockroach allergens and asthma. J Allergy Clin Immunol 107:419-428, 2001. | ||||
| 5. Pomés A, Vailes LD, Helm RM, Chapman MD. IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1. Eur J Biochem 269:3086-3092, 2002. | ||||
| 6. Nalyanya G, Gore JC, Linker HM, Schal C. German cockroach allergen levels in North Carolina schools: comparison of integrated pest management and conventional cockroach control. J Med Entomol 46:420-427, 2009. | ||||
| File Name | Size | Added | Link |
| EPC-BG2-X_CoA | 245.21KB | 19/02/2015 | Download |
| Certificate of Analysis for Bla g 2 ELISA 2.0 kit | |||
- Product Code: EPC-BG5-5
- Availability: In Stock
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$1,985.00
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Tags: bla g 5, ELISA 2.0, pre-coated plate, cockroach,
