Fel d 4 ELISA 2.0 kit - Single plate (EPC-FD4-1)

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*ELISA 2.0 kit for measuring Felis domesticus* allergen, Fel d 4.**
Antibodies:		5F3/pAb
Standard Range:		100-0.02ng/mL
Limit of Detection:		0.08ng/mL
Background:		OD<0.08 at 450nm
Coefficient of Determination:		R-squared>0.98
Contents:		Plate: Pre-coated with anti-Fel d 4 monoclonal antibody 5F3 Vial 1: (white top) Fel d 4 allergen standard Concentration: 100ng/ml Vial 2: (brown) Rabbit anti Fel d 4 antibody Vial 3: (blue top) Peroxidase-conjugated Goat Anti-Rabbit Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid)
Storage:		Store at 2-8°C
Expiry:		See product insert.
Not Provided:		Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required)
Notes:		The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:		EPC-FD4-1 Certificate of Analysis Validation Performance Data
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Pre-coated Plate:		96-well polystyrene microtiter plate coated with monoclonal antibody 5F3 and treated with stabilizing agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody:		5F3
Immunogen:		Fel d 4
Isotype:		Mouse IgG1
Specificity:		Binds to an epitope on cat Felis domesticus allergen, Fel d 4.
Purification:		Produced in vitro and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

		Certificate of Analysis
Detection Antibody:		Rabbit polyclonal antiserum
Immunogen:		Fel d 4
Isotype:		Multiple
Specificity:		The antiserum contains IgG antibodies to cat Felis domesticus allergen, as well as IgG antibodies to other cat allergens.
Activity:		Titrated for use in ELISA for Fel d 4 allergen at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free.

		Certificate of Analysis
Allergen Standard:		Recombinant Fel d 4 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration:		100ng/mL (based on amino acid analysis)
References: 1. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC, Chapman MD. Indoor allergens and asthma: report of the Third International Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24. 2. Platts-Mills TA, Vaughan J, Squillace S, Woodfolk J, Sporik R. Sensitisation, asthma, and a modified Th2 response in children exposed to cat allergen: a population-based cross-sectional study. Lancet 2001; 357(9258):752-756. 3. Heinrich J, Bedada GB, Zock JP, Chinn S, Norback D, Oliveri M et al. Cat allergen level: its determinants and relationships to specific IgE to cat across European centers. J Allergy Clin Immunol 2006; 118(3):674-681 4. Jarvis D, Zock JP, Heinrich J, Svanes C, Verlato G, Olivieri M et al. Cat and dust mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in adults. J Allergy Clin Immunol. 2007 Mar; 119(3):697-704.