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Lol p 1 ELISA 2.0 kit - Single plate (EPC-LP1-1)

  • Lol p 1 ELISA 2.0 kit - Single plate (EPC-LP1-1)
 

ELISA 2.0 kit for measuring Lolium perenne allergen, Lol p 1.
Antibodies: 5G7/8D10
Standard Range: 1,000-1.95 ng/mL
Limit of Detection: 3.91 ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Lol p 1 monoclonal antibody 5G7
Vial 1: (white top) Lol p 1 allergen standard
Concentration: 10,000 ng/ml
Vial 2: (brown) anti-Lol p 1 antibody 8D10
Vial 3: (blue top) Streptavidin-Peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: The ELISA 2.0 kit should be stored at 2-8°C.
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes:

The following extracts tested positive in this assay:

  • Lolium perenne (Rye grass)
  • Phleum pratense (Timothy grass)
  • Poa pratensis (Meadow fescue)
  • Dactylis glomerate (Cat grass)
  • Holcus lanatus (Velvet grass)

 

The following extracts tested negative in this assay:

  • Cynodon dactylon (Bermuda grass)
  • Secale cereal (Cultivated Rye Grass)

The allergen standard is recommended for immunoassay calibration purposes only.

A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-LP1-1 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plates coated with
monoclonal antibody 5G7 and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: 5G7
Immunogen: Lol p 1
Isotype: Mouse IgG1/Kappa
Specificity:

Binds to group 1 grass pollen allergens due to sequence homology

 
Purification: Produced in cell culture and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE.
  Certificate of Analysis
Detection Antibody: 8D10
Immunogen: Lol p 1
Isotype Mouse IgG1/Lamda
Specificity: Binds to group 1 grass pollen allergens due to sequence homology
Activity: Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.
  Certificate of Analysis
Allergen Standard: Purified natural Lol p 1 prepared in 1% BSA/30%
glycerol/PBS, pH 7.4.
Concentration/Calibration: 10,000 ng/mL (based on amino acid analysis)
References:  
 
 
 

 

 
File Name Size Added Link
EPC-LP1-X_CoA 1.04MB 28/04/2020 Download
Certificate of Analysis for Lol p 1 ELISA 2.0 kit
  • Product Code: EPC-LP1-1
  • Availability: In Stock

  • $545.00

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Tags: Lol p 1, ELISA 2.0, pre-coated plate,