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Mus m 1 ELISA 2.0 kit - Twenty-five plate pack (EPC-MM1-25)

  • Mus m 1 ELISA 2.0 kit - Twenty-five plate pack (EPC-MM1-25)

ELISA 2.0 kit for measuring Mouse Urinary Protein (MUP) allergen, Mus m 1.

Antibodies: pAb/pAb
Standard Range: 25-0.05 ng/mL
Limit of Detection: 0.19ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:

Plate: Pre-coated with anti-Mus m 1 polyclonal rabbit capture antibody.
Vial 1: (white top) Mus m 1 allergen standard
Concentration: 250 ng/mL
Vial 2: (brown) Biotinylated polyclonal rabbit anti-Mus m 1 antibody.
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)

Storage: Store at 2-8°C
Expiry: See product insert.
Not Provided:

Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)

Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:

EPC-MM1-25 Certificate of Analysis

Validation Performance Data

For research and commercial use in vitro: not for human in vivo or therapeutic use.

   
  Certificate of Analysis
Pre-coated Plate: 96-well polystyrene microtiter plate coated with
Rabbit polyclonal antiserum and treated with stabilizing
agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody: Rabbit polyclonal antiserum
Immunogen: Mus m 1
Isotype: Multiple
Specificity: Binds to an epitope on mouse Mus musculus urinary protein allergen, Mus m 1.
Purification: Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

  Certificate of Analysis
Detection Antibody: Rabbit polyclonal antiserum
Immunogen: Mus m 1
Isotype: Multiple
Specificity: Binds to an epitope on mouse Mus musculus urinary protein allergen, Mus m 1.
Purification: Affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.
Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.

  Certificate of Analysis
Allergen Standard: Purified natural Mus m 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration: 250 ng/mL (based on amino acid analysis)

References: 

1. Phipatanakul W, Eggleston PA, Wright EC, Wood RA.  Mouse allergen. I. The prevalence of mouse allergen in inner-city homes. The National Cooperative Inner-City Asthma Study.  J Allergy Clin Immunol. 2000;106:1070-4. 

2. Phipatanakul W, Eggleston PA, Wright EC, Wood RA.  Mouse allergen. II. The relationship of mouse allergen exposure to mouse sensitization and asthma morbidity in inner-city children with asthma.  J Allergy Clin Immunol. 2000;106:1075-80.

3. Permaul P, Hoffman E, Fu C, Sheehan W, Baxi S, Gaffin J, Lane J, Bailey A, King E, Chapman M, Gold D, Phipatanakul W.  Allergens in urban schools and homes of children with asthma.  Pediatr Allergy Immunol. 2012;23:543-9.

4.  Ahluwalia SK, Peng RD, Breysse PN, Diette GB, Curtin-Brosnan J, Aloe C, Matsui EC.  Mouse allergen is the major allergen of public health relevance in Baltimore City.  J Allergy Clin Immunol. 2013;132:830-5.

5.  Ownby DR.  Will the real inner-city allergen please stand up?  J Allergy Clin Immunol. 2013;132:836-7.

 

 

File Name Size Added Link
EPC-MM1-X_CoA 1.12MB 19/02/2015 Download
Certificate of Analysis for Mus m 1 ELISA 2.0 kit
  • Product Code: EPC-MM1-25
  • Availability: In Stock
  • $9,095.00

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Tags: mus m 1, ELISA 2.0, pre-coated plate, mouse allergen,