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ELISA 2.0 kit for measuring Canis familiaris allergen, Can f 1. |
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Antibodies: | 10D4/pAb | ||||||
Standard Range: | 50-0.1 ng/mL | ||||||
Limit of Detection: | 0.39ng/mL | ||||||
Background: | OD<0.08 at 450nm | ||||||
Coefficient of Determination: | R-squared>0.98 | ||||||
Contents: |
Plate: Pre-coated with anti-Can f 1 monoclonal antibody 10D4 |
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Storage: | The ELISA 2.0 kit should be stored at 2-8°C. | ||||||
Expiry: | See product insert. | ||||||
Not Provided: |
Type I ultrapure water or 18.2MΩ de-ionized water |
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Notes: | The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. |
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Product Resources: | |||||||
For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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Certificate of Analysis | |||||||
Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 10D4 and treated with stabilizing agent. Sealed in foil pouch with desiccant. |
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Monoclonal Antibody: | 10D4 (clone 10D4 C4 A10) | ||||||
Immunogen: | Can f 1 | ||||||
Isotype: | Mouse IgG2a/kappa | ||||||
Specificity: | Binds to an epitope on dog Canis familiaris allergen, Can f 1. | ||||||
Purification: | Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | ||||||
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Certificate of Analysis | |||||||
Detection Antibody: | Rabbit polyclonal antiserum | ||||||
Immunogen: | Dog fur extract | ||||||
Isotype | Multiple | ||||||
Specificity: | The antiserum contains IgG antibodies to dog Canis familiaris allergen, Can f 1, as well as IgG antibodies to other dog allergens.. |
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Purification: | Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | ||||||
Activity: | Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free. | ||||||
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Certificate of Analysis | |||||||
Allergen Standard: | Purified natural Can f 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. | ||||||
Concentration/Calibration: | 500 ng/mL (based on amino acid analysis) | ||||||
References: | |||||||
1. Larsen JN, Ford A, Gjesing B, et al. The collaborative study of the international standard of dog, Canis domesticus, hair/dander extract. J Allergy Clin Immunol 1988; 82:318-330. | |||||||
2. DeGroot H, Goei K, Van Sicter P, et al. Affinity purification of a major and minor allergen from dog extract: Serologic activity of affinity-purified Can f 1 and Can f 1-depleted extract. J Allergy Clin Immunol 1991; 87:1056-61. | |||||||
3. Schou C, Hansen G, Linter T, et al. Assay for the major dog allergen, Can f 1 investigation of house dust samples and commercial dog extracts. J Allergy Clin Immunol 1992; 88:847-53. | |||||||
4. Ingram JM, Sporik R, Rose G, Honsiger R, Chapman MD, Platts-Mills TAE. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: Relationship to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96:449-456. | |||||||
5. Sporik RB, Ingram JM, Price W, Sussman JH, Honsiger RW, Platts-Mills TAE. Association of asthma with serum IgE and skin test reactivity to allergens among children living at high altitude: Tickling the dragon’s breath. Amer J Resp Crit Care Med 1995;151:1388-1392. | |||||||
File Name | Size | Added | Link |
EPC-CF1-X_CoA | 228.99KB | 19/02/2015 | Download |
Certificate of Analysis for Can f 1 ELISA 2.0 kit |
Tags: can f 1, ELISA 2.0, pre-coated plate,