Can f 1 ELISA 2.0 kit - Five plate pack (EPC-CF1-5)

Description
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ELISA 2.0 kit for measuring Canis familiaris allergen, Can f 1
Antibodies:		10D4/pAb
Standard Range:		50-0.1 ng/mL
Limit of Detection:		0.39ng/mL
Background:		OD<0.08 at 450nm
Coefficient of Determination:		R-squared>0.98
Contents:		Plate: Pre-coated with anti-Can f 1 monoclonal antibody 10D4 Vial 1: (white top) Can f 1 allergen standard Concentration: 500 ng/ml Vial 2: (brown) Rabbit anti Can f 1 antibody Vial 3: (blue top) Peroxidase-conjugated Goat Anti-Rabbit Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid)
Storage:		The ELISA 2.0 kit should be stored at 2-8°C.
Expiry:		See product insert.
Not Provided:		Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required)
Notes:		The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.
Product Resources:		EPC-CF1-5 Certificate of Analysis Validation Performance Data
For research and commercial use in vitro: not for human in vivo or therapeutic use.
		Certificate of Analysis
Pre-coated Plate:		96-well polystyrene microtiter plate coated with monoclonal antibody 10D4 and treated with stabilizing agent. Sealed in foil pouch with desiccant.
Monoclonal Antibody:		10D4 (clone 10D4 C4 A10)
Immunogen:		Can f 1
Isotype:		Mouse IgG2a/kappa
Specificity:		Binds to an epitope on dog Canis familiaris allergen, Can f 1
Purification:		Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.

		Certificate of Analysis
Detection Antibody:		Rabbit polyclonal antiserum
Immunogen:		Dog fur extract
Isotype		Multiple
Specificity:		The antiserum contains IgG antibodies to dog Canis familiaris allergen, Can f 1, as well as IgG antibodies to other dog allergens.
Purification:		Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Activity:		Titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22 μm filtered, preservative free.

		Certificate of Analysis
Allergen Standard:		Purified natural Can f 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4.
Concentration/Calibration:		500 ng/mL (based on amino acid analysis)
References:
1. Larsen JN, Ford A, Gjesing B, et al. The collaborative study of the international standard of dog, Canis domesticus, hair/dander extract. J Allergy Clin Immunol 1988; 82:318-330.
2. DeGroot H, Goei K, Van Sicter P, et al. Affinity purification of a major and minor allergen from dog extract: Serologic activity of affinity-purified Can f 1 and Can f 1-depleted extract. J Allergy Clin Immunol 1991; 87:1056-61.
3. Schou C, Hansen G, Linter T, et al. Assay for the major dog allergen, Can f 1 investigation of house dust samples and commercial dog extracts. J Allergy Clin Immunol 1992; 88:847-53.
4. Ingram JM, Sporik R, Rose G, Honsiger R, Chapman MD, Platts-Mills TAE. Quantitative assessment of exposure to dog (Can f 1) and cat (Fel d 1) allergens: Relationship to sensitization and asthma among children living in Los Alamos, New Mexico. J Allergy Clin Immunol 1995; 96:449-456.
5. Sporik RB, Ingram JM, Price W, Sussman JH, Honsiger RW, Platts-Mills TAE. Association of asthma with serum IgE and skin test reactivity to allergens among children living at high altitude: Tickling the dragon’s breath. Amer J Resp Crit Care Med 1995;151:1388-1392.