Natural Der p 2 Molecular Reference Standard (MRS-NDP2)

From spent mite culture by multi-step affinity chromatography. 

Preservative-free and carrier-free in phosphate buffered
saline, pH 7.4. Filtered through 0.22µ filter.

• Allow vial to reach room temperature before use
• Tap vial gently to collect all material at the bottom
• Using a sterile syringe reconstitute the MRS to desired concentration by injecting a suitable volume of a buffer of choice (e.g. PBS, pH 7.4 or 1% BSA/50% glycerol/PBS, pH 7.4). 
• Mix by gently swirling the vial until content is completely dissolved. 
• Adding 1ml of buffer will result in a Der p 2 concentration of 10,000ng/ml. 

1. Smith AM et al. The molecular basis of antigenic cross-reactivity between the group 2 mite allergens.  J Allergy Clin Immunol 2001;107:977-84.
2. Derewenda U et al.The crystal structure of a major dust mite allergen Der p 2, and its biological implications. J Mol Biol. 2002;318:189-97.
3. Trompette A et al. Allergenicity resulting from functional mimicry of a Toll-like receptor complex protein. Nature. 2009;457:585-8. 
4. Chapman MD et al. The European Union CREATE project: a model for international standardization of allergy diagnostics and vaccines. J Allergy Clin Immunol. 2008;122:882-889.
5. van Ree R et al. The CREATE project: development of certified reference materials for allergenic products and validation of methods for their quantification. Allergy. 2008;63(3):310-26.
6. Kaul S et al. Regulatory environment for allergen-specific immunotherapy. Allergy 2011;66:753-64.
7. Chapman MD and Briza P. Molecular approaches to allergen standardization. Curr Allergy Asthma Rep. 2012;12:478-84.
8. Chapman MD et al. Technological Innovations for High-Throughput Approaches to In Vitro Allergy Diagnosis. Curr Allergy Asthma Rep. 2015;15:36