Recombinant Der p 2 (RP-DP2C-1)

Description
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Allergen:	rDer p 2 (Dermatophagoides pteronyssinus allergen 2.0103), house dust mite
Unit:	250µg
Source:	P. pastoris
Mol. Wt:	14kD
Purification:	Purified from Pichia pastoris culture supernatant by affinity chromatography. Purity on silver stained SDS-PAGE > 95%.
Concentration:	See product insert.
Formulation:	Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22 µm filter.
Storage:	Store at -20ºC.
Notes:	(1) rDer p 2.0103 is a Der p 2 isoform having 4 amino acid substitiutions different from Der p 2 0101. It does react in the Group 2 mite ELISA. (2) Not tested for endotoxin.
Genbank:
PDB:	1KTJ X-ray crystal structure; 1A9V NMR structure
Product Resources:	Recombinant Der p 2.0103 Certificate of Analysis
Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use.
References:
Heyman PW, Chapman MD, Fox J, Aalberse, RC, PlattsMills, TAE. Antigenic and structural analysis of the Group II allergens (Derp II and Der f II) from house dust mites (Dermatophagoides sp). J Allergy Clin Immunology 1989;83:1055-67. Mueller GA, Smith AM, Williams DC Jr., Hakkaart GAJ, Aalberse RC, Chapman MD, Rule, GS, Benjmin DC. Expression and secondary structure determination by NMR methods of the major house dust mite allergen Der p 2. J Biol Chem 1997;272:26893-8. Smith AM, Benjamin DC, Hozic N, Derewenda U, Smith WA, Thomas WR, Gafvelin G, van Hage-Hamsten M, Chapman MD. The molecular basis of antigenic cross-reactivity between the group 2 mite allergens. J Allergy Clin Immunol 2001;107:977-84. Mueller GA, Smith AM, Chapman MD, Rule GS, Benjamin DC. Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2. J Biol Chem 2001;276:9359-65.