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Recombinant Bla g 1 (RP-BG1-1)

  • Recombinant Bla g 1 (RP-BG1-1)
Allergen: rBla g 1 (Blattella germanica allergen 1.0101 ), German cockroach
Unit: 250µg
Source: P. pastoris
Mol. Wt: Multiple molecular forms (see Notes)
Purification: Purified from Pichia pastoris culture by affinity chromatography. Purity >95% by SDS-PAGE.
Concentration:

See product insert. 

Formulation:

Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22 µm filter.

Storage: Store at -20ºC.
Notes: (1) rBla g 1 is produced in P. pastoris. As a result of proteolytic cleavage of tandem amino acid repeats, the product contains multiple molecular forms of rBla g 1. (2) Not tested for endotoxin.
Genbank:

Genbank: AF072219

PDB:  
Product Resources: Recombinant Bla g 1 Certificate of Analysis
Related products:

EL-BG1 Bla g 1 ELISA
ST-BG1 Bla g 1 Standard
MA-10A6-1 10A6 anti Bla g 1
PA-BG1-1 Rabbit anti Bla g 1
PA-PAE-1 Rabbit anti P. americana

Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.

References:

  1. Pomés A, Melén E, Vailes LD, Reteif JD, Arruda LK, Chapman MD. Novel allergen structures with tandem amino acid repeats derived from German and American cockroach. J Biol Chem 1998;273:30801-7.
  2. Pomés A, Smith AM, Grégoire C, Vailes LD, Arruda LK, Chapman MD. Functional properties of cloned allergens from dust mite, cockroach, and cat- are they relevant to allergenicity? ACI Int. 2001;13:162-169. >Pomés A, Vailes LD, Helm RM, Chapman MD. Ige reactivity of tandem repeats derived from cockroach allergen, Bla g 1. Eur J Biochem 2002;269:3086-92 Gore JC, Schal C. Gene expression and tissue distribution of the major human allergen Bla g 1 in the German cockroach, Blattella germanica L. (Dictyoptera: Blattellidae). J Med Entomol, 2004;41:953-960.
  3. Gore JC, Schal C. Expression, production and excretion of Bla g 1, a major human allergen, in relation to food intake in the German cockroach, Blattella germanica. Med Vet Entomol, 2005;19:127-134
  4. Filep S, Tsay A, Vailes LD, Gadermaier G, Ferreira F, Matsui E, King E, Chapman MD. A multi-allergen standard for the calibration of immunoassays: CREATE principles applied to eight purified allergens. Allergy 2012;67:235–241. PMID: 22092159, PMCID: PMC3257384 [Available on 2013/2/1].
File Name Size Added Link
RP-BG1_CoA 45.91KB 20/05/2014 Download
Recombinant Bla g 1 Certificate of Analysis
  • Product Code: RP-BG1-1
  • Availability: In Stock
  • $625.00

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