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Der f 1 ELISA 2.0 kit - Five plate pack (EPC-DF1-5)
| ELISA 2.0 kit for measuring D. farinae allergen, Der f 1. | ||||
| Antibodies: | 6A8/4C1 | |||
| Standard Range: | 50-0.1 ng/ml | |||
| Limit of Detection: | 0.19ng/ml | |||
| Background | OD<0.08 at 450nm | |||
| Coefficient of Determination: | R-squared>0.98 | |||
| Contents: | Plate: Pre-coated with anti-Der f 1 monoclonal antibody 6A8 Vial 1: (white top) Der f 1 allergen standard Concentration: 500 ng/ml Vial 2: (brown) Biotinylated monoclonal antibody 4C1 Vial 3: (blue top) Streptavidin-peroxidase Bottle 1: Wash buffer, (10x concentrate) Bottle 2: Assay buffer, (10x concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid) |
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| Storage: | Store at 2-8°C. | |||
| Expiry: | See product insert. | |||
| Not Provided: | Type I ultrapure water or 18.2MΩ de-ionized water Volumetric measuring equipment (e.g. serological pipette, graduated cylinder) Clean containers for buffer and reagent preparation Calibrated single and multi-channel micropipettes and tips Vortex mixer Plate reader capable of reading absorbance at 450nm Analysis software (recommended, but not required) |
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| Notes: | The allergen standard is recommended for immunoassay calibration purposes only. A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website. |
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| Product Resources: | ||||
For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis | ||||
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 6A8 and treated with stabilizing agent. Sealed in foil pouch with desiccant. |
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| Monoclonal Antibody: | 6A8 (clone 6A8 B10 D12) | |||
| Immunogen: | Der f 1 | |||
| Isotype: | Mouse IgG1 | |||
| Specificity: | Binds to a species specific epitope on dust mite Dermatophagoides farinae allergen, Der f 1. | |||
| Purification: | Produced in ascites and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. | |||
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| Certificate of Analysis | ||||
| Detection Antibody: | 4C1 (clone 4C1 B8 3F8) | |||
| Immunogen: | Der f 1 | |||
| Isotype: | Mouse IgG1 | |||
| Specificity: | Binds to anepitope present on dust mite Dermatophagoides Group 1 allergens (Der f 1, Der p 1, Der m 1, Eur m 1). | |||
| Purification: | Produced in ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. | |||
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free. | |||
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| Certificate of Analysis | ||||
| Allergen Standard: | Purified natural Der f 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. |
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| Concentration: | 500 ng/mL (based on amino acid analysis) | |||
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References: 1. Chapman, M.D., Sutherland, W.M., and Platts-Mills, T.A.E. (1984). Recognition of two Dermatophagoides pteronyssinus specific epitopes on Antigen P1 using monoclonal antibodies: Binding to each epitope can be inhibited be sera from mite allergic patients. J. Immunol. 133:2488-2495. 2. Ford, A.W., Rawle, F.C., Lind, P., Spieksma, F.Th.M., Lowenstein, H. and Platts-Mills, T.A.E. (1985). Standardization of Dermatophagoides pteronyssinus: Assessment of potency and allergen content in ten coded extracts. Int. Archs. Allergy Appl. Immunol. 76:58-68. 3. Heymann, P.W., Chapman, M.D. and Platts-Mills, T.A.E. (1986). Antigen Der f 1 from the dust mite Dermatophagoides farinae: Structural comparison with Der p 1 from D. pteronyssinus and epitope specificity of murine IgG and human IgE antibodies. J. Immunol. 137:2841-2847. 4. Chapman, M.D., Heyman, P.W., Wilkins, S.R., Brown, M.J., and Platts-Mills, T.A.E. (1987). Monoclonal immunoassays for major dust mite (Dermatophagoides) allergens, Der p 1 and Der f 1, and quantitative analysis of the allergen content of mite and house dust extracts. J. Allergy Clin. Immunol. 80:184-194. 5. Platts-Mills, T.A.E. and Chapman, M.D. (1987). Dust mites: Immunology, allergic disease and environmental control. J. Allergy Clin. Immunol. 80:755-775 6. Chapman, M.D., Heymann, P.W., and Platts-Mills, T.A.E. (1987). Epitope mapping of two major inhalant allergens, Der p 1 and Der f 1, from mites of the genus Dermatophagoides. J. Immunol. 139: 1479-1484. 7. Chapman, M.D. (1988). Allergen specific monoclonal antibodies: New tools for the management of allerigic disease. Allergy 43 (5):7-14. 8. Luczynska, C.M., Arruda, L.K., Platts-Mills, T.A.E., Miller, J.D., Lopez, M., and Chapman, M.D. (1989). A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p 1 and Der f 1. J. Immunol. Meths. 118:227-235. 9. Platts-Mills T.A.E., Thomas W.R., Aalberse R.C., Vervloet D. and Chapman M.D. (1992). Dust mite allergens and asthma: Report of a second international workshop. J Allergy Clin Immunol 89:1046-1060. 10. Platts-Mills T.A.E., Vervloet D., Thomas W.R., Aalberse R.C., Chapman M.D. Indoor Allergens and Asthma: Report of the third international workshop. J Allergy Clin Immunol, 1997; 100:S1-S24. |
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| File Name | Size | Added | Link |
| EPC-DF1-X_CoA | 1.15MB | 19/02/2015 | Download |
| Certificate of Analysis for Der f 1 ELISA 2.0 kit | |||
- Product Code: EPC-DF1-5
- Availability: In Stock
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$1,985.00
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Tags: der f 1, ELISA 2.0. pre-coated plate, dust mite,
