Natural Der f 1 Molecular Reference Standard (MRS-NDF1)
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The Natural Der f 1 MRS is intended to serve as reference standard to determine the Der f 1 content of allergen preparations from house dust mite (Dermatophagoides farinae) by immunoassay |
| Allergen: |
NNatural Der f 1 (Dermatophagoides farinae allergen 1) |
| Source: |
D. farinae culture |
| Mol. Wt: |
24 kD |
| Purification: |
From spent mite culture by multi-step affinity chromatography.
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| Composition: |
10µg natural Der f 1, determined by Amino Acid Analysis, freeze dried in sealed glass vial. |
| SDS-PAGE: |
See inset. Silver-stained SDS-PAGE under non-reducing conditions shows a single band at 24 kD. |
| ELISA: |
Immunoreactive in Der f 1 specific ELISA. No trace contamination with Der f 2 was detected by ELISA. |
| Purity: |
>95% purity by in-solution LC-MS/MS after tryptic digest. |
| Formulation: |
Prior to lyophilization, natural Der f 1 was adjusted to 50 mM volatile ammonium bicarbonate with 3% trehalose.
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| Storage: |
Store at -20ºC |
| Product Resources: |
Natural Der f 1 Molecular Reference Standard Certificate of Analysis |
| The Natural Der f 1 MRS is an InbioTM product. |
| Allergens are provided for research and commercial use in vitro: not for human in vivo or therapeutic use. |
| Reconstitution: |
- Allow vial to reach room temperature before use
- Tap vial gently to collect all material at the bottom
- Using a sterile syringe reconstitute the MRS to desired concentration by injecting a suitable volume of a buffer of choice (e.g. PBS, pH 7.4 or 1% BSA/50% glycerol/PBS, pH 7.4).
- Mix by gently swirling the vial until content is completely dissolved.
- Adding 1ml of buffer will result in a Der f 1 concentration of 10,000ng/ml.
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| References: |
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- Lombardero M et al. Conformational stability of B cell epitopes on Group I and Group II Dermatophagoides spp. allergens. Effect of thermal and chemical denaturation on the binding of murine IgG and human IgE antibodies. J Immunol 1990; 144:1353-1360.
- Schultz O et al. A sensitive fluorescent assay for measuring the cysteine protease activity of Der p 1, a major allergen from the dust mite Dermataphagoides pteronyssinus. J Clin Pathol: Mol Pathol 1998;51:222-231.
- Chapman MD et al. The European Union CREATE project: a model for international standardization of allergy diagnostics and vaccines. J Allergy Clin Immunol. 2008;122:882-889.
- van Ree R et al. The CREATE project: development of certified reference materials for allergenic products and validation of methods for their quantification. Allergy. 2008;63(3):310-26.
- Kaul S et al. Regulatory environment for allergen-specific immunotherapy. Allergy 2011;66:753-64.
- Chapman MD and Briza P. Molecular approaches to allergen standardization. Curr Allergy Asthma Rep. 2012;12:478-84.
- Chapman MD et al. Technological Innovations for High-Throughput Approaches to In Vitro Allergy Diagnosis. Curr Allergy Asthma Rep. 2015;15:36.
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| File Name |
Size |
Added |
Link |
| MRS-NDF1_CoA |
197.02KB |
24/01/2017 |
Download |
| Natural Der f 1 Molecular Reference Standard Certificate of Analysis |
- Product Code: MRS-NDF1
- Availability: In Stock
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