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Recombinant Der f 1 (RP-DF1-1)

  • Recombinant Der f 1 (RP-DF1-1)
Allergen: rDer f 1 (Dermatophagoides farinae allergen 1), house dust mite
Unit: 250µg
Source: Pichia pastoris
Mol. Wt: ~36-40kD
Purification:

Purified from Pichia pastoris culture by mAb affinity chromatography. Purity >95% by SDS-PAGE.

Concentration:

See product insert. 

Formulation:

Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22µm filter.

Storage: Store at -20ºC
Notes: (1) rDer f 1 produced in P. pastoris is glycosylated and has a higher MW than the natural allergen. The doublet is due to dimer formation.
Genbank: 3D6S X-ray crystal structure
PDB:  
Product Resources: Recombinant Der f 1 Certificate of Analysis
Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.
References:  
  1. Best EA, Stedman KE, Bozic CM, Hunter SW, Vailes LD, Chapman MD, McCall CA, McDermott MJ. A recombinant group 1 house dust mite allergen. rDer f 1, with biological activities similar to those of the native allergen. Prot Exp Pur 2000; 20:462-471.
  2. Chapman MD, Smith AM, Vailes LD, Arruda LK, Dhanaraj V, Pomés A. Recombinant allergens for diagnosis and therapy of allergic disease. J Allergy Clin Immunol 2000; 106:409-418.
  3. Chruszcz M, Chapman MD, Vailes LD, Stura EA, Saint-Remy JM, Minor W, Pomés A. Crystal structures of mite allergens Der f 1 and Der p 1 reveal differences in surface-exposed residues that may influence antibody binding. J Mol Biol, 2009;386:520-530.
File Name Size Added Link
RP-DF1_CoA 34.65KB 20/05/2014 Download
Recombinant Der f 1 Certificate of Analysis
  • Product Code: RP-DF1-1
  • Availability: In Stock
  • $625.00

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